In Vitro Plant Regeneration from Stem Explants of Phaseolus vulgaris L

In vitro regeneration of shoot buds and roots from stem explants of the French bean, isolated from 5-6-day-old seedlings was studied under defined cultural conditions. Stem explants were cultured on MS medium supplemented with various concentrations of auxins and cytokinins. Among various concentrations evaluated, BAP (2.0 mg/l) + 2-ip (2.0 mg/l) + 2,4-D (1.0 mg/l) resulted in callus initiation within a week, which extended over the surface of the explant followed by the induction of shoot buds within 3 weeks of inoculation. Elongation of shoots was readily achieved with reduced concentrations of the adjuvant. Rooting was obtained with MS medium supplemented with GA3 (0.25 mg/l) and IBA (0.25 mg/l). The present study deals with the development of a protocol for callus mediated regeneration from stem explants in Phaseolus vulgaris.