SYNERGISTIC ANTI INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF CHLOROGENIC ACID AND L – THEANINE

Aim: To analyse the synergistic effect of chlorogenic acid and L theanine on antioxidant and anti inflammatory activity.

Introduction: Chlorogenic acid ,an ester of caffeic acid and quinic acid is a major phenolic compound in coffee ,daily intake in coffee drinkers is 0.5-1g .Chlorogenic acid and caffeic acid are antioxidants in vitro and might therefore contribute to the prevention of cardiovascular disease. The antioxidant activities are due to phenolic compounds which are important in biological systems because of their production of ROS which may be related to degenerative disease processes such as DNA damage, lipid peroxidation etc. L- theanine is a water soluble amino acid isolated from green tea. Camellia sinensis teas have attracted a great deal of attention due to their numerous health benefits, including antioxidant, hypoglycaemic, anti carcinogenic, antimicrobial, antiinflammatory activities.

Materials and Methods: Purified compounds of chlorogenic acid and L- Theanine were used. To find the antioxidant activity, DPPH radical scavenging was used and to find the anti inflammatory activity, albumin denaturation inhibition assay was used.

Results and Discussion: The compound showed both antioxidant and anti inflammatory activity in a concentration dependent manner. Therefore the synergistic effect exhibits antioxidant and anti inflammatory activity.