ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESISED USING GRAPE SEED EXTRACT: AN in vitro STUDY

Background: Zinc oxide nanoparticles have received much attention recently due to its properties such as its extensive antimicrobial activity, eco-friendliness, and simplicity. ZnO especially has special attention due to its excellent antibacterial nature. In addition to these properties, Zinc oxide nanoparticles were also found to be less toxic in in-vitro tests. In the current study we have chosen grape seed due to its protection against oxidative damage, and anti-cholesterol, anti-diabetic and antiplatelet properties.

Aim: The aim of the current study was to analyse the antioxidant activity of grape seed mediated zinc oxide nanoparticles.

Materials and Methods: Collected grape seeds were powdered and stored in an air-sealed container. 1g of grape seed extract was mixed with 100 ml of distilled water and is boiled for 15 minutes. This solution is then filtered, to which 20 ml distilled water is added. To this solution 0.862 g of zinc oxide is added and was subjected to constant stirring untitled the formation of white suspension. This is centrifuged for 10 minutes and the antioxidant property against DPPH free radical scavenger is assessed.

Results: The percentage inhibition was only 43.8% when the concentration was 5 μl, at 10 μl it was 46.4%, at 15 μl it was 49.9%, at 20 μl it was 58.3% and at 25 μl it was 60.2%. It was observed from the spectra that the extract at 517 nm had highest radical scavenging activity at a concentration of 25 μl (60.2%), which is indicative of significant antioxidant activity as potent as DPPH itself.

Conclusion: The zinc oxide nanoparticles synthesized using the grape seed extract acts as a potent antioxidant agent with least side effects. Further clinical trials can be conducted in the future and can be used as a replacement to the commercially available drugs.

Background: Zinc oxide nanoparticles have received much attention recently due to its properties such as its extensive antimicrobial activity, eco-friendliness, and simplicity. ZnO especially has special attention due to its excellent antibacterial nature. In addition to these properties, Zinc oxide nanoparticles were also found to be less toxic in in-vitro tests. In the current study we have chosen grape seed due to its protection against oxidative damage, and anti-cholesterol, anti-diabetic and antiplatelet properties.

Aim: The aim of the current study was to analyse the antioxidant activity of grape seed mediated zinc oxide nanoparticles.

Materials and Methods: Collected grape seeds were powdered and stored in an air-sealed container. 1g of grape seed extract was mixed with 100 ml of distilled water and is boiled for 15 minutes. This solution is then filtered, to which 20 ml distilled water is added. To this solution 0.862 g of zinc oxide is added and was subjected to constant stirring untitled the formation of white suspension. This is centrifuged for 10 minutes and the antioxidant property against DPPH free radical scavenger is assessed.

Results: The percentage inhibition was only 43.8% when the concentration was 5 μl, at 10 μl it was 46.4%, at 15 μl it was 49.9%, at 20 μl it was 58.3% and at 25 μl it was 60.2%. It was observed from the spectra that the extract at 517 nm had highest radical scavenging activity at a concentration of 25 μl (60.2%), which is indicative of significant antioxidant activity as potent as DPPH itself.

Conclusion: The zinc oxide nanoparticles synthesized using the grape seed extract acts as a potent antioxidant agent with least side effects. Further clinical trials can be conducted in the future and can be used as a replacement to the commercially available drugs.